Bulk RNA-seq
Genome-wide transcriptional profiling using bulk RNA sequencing
Bulk RNA sequencing enables quantitative profiling of gene expression across samples, providing a robust view of transcriptional changes associated with biological conditions or experimental perturbations. Standardised workflows support reproducible processing of RNA-seq data, from raw reads through to expression quantification, while maintaining compatibility with widely used downstream analysis approaches.
Careful processing and quality control of RNA-seq datasets allows reliable comparison of expression levels between samples, supporting differential expression analysis, pathway interrogation, and functional interpretation. Re-analysing existing RNA-seq data can extract additional biological insight and ensure results are generated using current best-practice methods without the need for new sequencing.
€ Priced based on project discussion(s). Get in touch:
Details
This service is for the FASTQ data processing, QC reports, and minimal biological or statistical interpretation.
Optional add-ons such as figure preparation, or data upload to public repositories, are available on request.
Project Setup
• Includes project discussions and data transfers
Analysis per Sample
• Suitable for 1 to >100 samples
NCBI Submission
• FASTQ FTP upload to generate BioProject and Accession IDs
Figures & Statistics
• Composition, diversity, statistical test, and differential abundance
Video Discussions – n=3
• Kick-off, update, and wrap-up calls
Turnaround Time – 2–3 weeks
• Queue and project size dependent
Delivery – Dropbox / FileZilla
• Data retained for 30 days
Deliverables
All files are shared via secure, GDPR-compliant data transfers.
Data upload and release will be organised via Dropbox or Filezilla.
Compute resources are provided by an Irish-based cloud computing provider, CloudCIX.
multiqc_report.html
• Aggregated quality control summary of raw and processed sequencing data
aligned_reads
• Processed sequencing reads or transcriptome mappings used for expression estimation
expression_matrix.csv
• Quantitative gene or transcript expression values (rows) per sample (columns)
normalised_expression_matrix.csv
• Normalised expression values suitable for between-sample comparison
sample_qc_metrics.csv
• Per-sample sequencing and mapping statistics
Requirements
Pipelines can be run on (i) newly generated datasets, (ii) publicly available metagenomes, or (iii) a combination of both, enabling comparative analyses across studies and environments.
This flexibility allows integration of novel samples with existing microbiome resources for broader context and reproducibility.
Input Format
• Paired- or single-end FASTQ (gzipped)
Read Length
• 50–150 bp
Depth
• ≥ 3 Mn reads
Optional Preprocessing
• Read repair – BBTools
Accepted Platforms
• Illumina, MGI, Element Biosciences, Ultima Genomics
Technical
All analyses are executed within isolated Conda environments, ensuring full reproducibility and dependency control across runs.
Each tool and database version is tracked, guaranteeing consistent results between projects and over time.
QC
• Tool(s): FastQC v0.12
• Purpose: Per-sample read quality analysis
Reporting
• Tool(s): MultiQC v1.31
• Purpose: Combined QC report
Trimming
• Tool(s): fastp v0.23
• Purpose: Adapter and low-quality trimming
Host Removal
• Tool(s): Kraken2 v2.0.8
• Purpose: Optional; host genome or taxonomy database required
Deduplication
• Tool(s): Clumpify v39.33
• Purpose: Duplicate read removal
Read Mapping
• Tool(s): Bowtie2
• Purpose: Short-read mapping to known databases
Workflow
Client data are transferred securely to BioFigR via Dropbox/Filezilla and processed on CloudCIX infrastructure using rsync. All transfers occur within GDPR-compliant environments, with optional deposition to NCBI via FTP only upon client approval. No data are shared or stored beyond the agreed workflow stages.
Contact BioFigR
This streamlined, reproducible nf-core/rnaseq pipeline ensures data integrity from upload to analysis. BioFigR provides transparent, compliant handling at every stage—so clients can focus on results, not logistics.
Contact BioFigR with the number of samples and reads per sample to receive a quotation, or to discuss project requirements.